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The calculation of % inhibition was carried out via following equation.Where, Ab = absorbance of the blank (DPPH solution without sample) and As = absorbance of the sample (DPPH solution with quercetin)
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(major emphasis is Phase I metabolism – very thorough coverage) Smith, Glucuronidation, UNC Chapel Hill, 9/2011 Conjugation Reactions – Some Definitions Glucuronidation “conjugation” is the addition of a molecule to the drug or xenobiotic GSH conjugation GSH = glutathione (a tripeptide derivative) Sulfate conjugation Amino acid conjugation Acetylation Methylation “Phase II metabolism” is terminology coined by RT Williams, whereby a compound is first subject to oxidation reduction or hydrolysis (Phase I reactions), which may be associated with bioactivation, and then the functional group created is conjugated to a less toxic or inactive compound